Freeze grinder JXFSTPRP-II grinding broken mouse joint ligament experiment - Clinical Medicine Articles - China Articles

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I. Research Background Mouse joints have strong toughness and hard bones. Manual grinding is usually difficult to grind; it is even more difficult to handle high-throughput samples. In this experimental scheme, the jelly specimen JXFSTPRP-II of Shanghai Netcom was taken as an example, and the mouse joint samples were ground and processed, and the samples were processed in 45 seconds.

Second, the grinding method
1. Experimental steps:
a, prepare 100 mg of mouse joint ligament to be ground, a set of 5 ml steel cans to be cleaned and sterilized, several grinding beads, one liter of liquid nitrogen;
b, the sample and the grinding beads were placed in a 5 ml steel can, and the whole was immersed in liquid nitrogen for 1 min, and then mounted on a Shanghai Jingxin frozen grinder;
c, set the frequency to 60hz, time 45s, start the equipment for grinding;
d, after the equipment stops running, take out the grinding steel tank;
e, open the steel tank and add 1ml of extract to the inside to mix manually;
f, continue the subsequent extraction experiment operation.

Grinding experiment results:

Sample before grinding

After the sample is ground