Chlamydia pneumoniae using Elisa detection - Database & Sql Blog Articles

EL-C1600N100013-B

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

From

Jiangsu Fia

Summary of the study: Chlamydia pneumoniae infection is widespread in the world, with recessive infections as the mainstay. Its clinical manifestations are diverse, mainly causing atypical pneumonia, and can also cause pharyngitis, bronchitis, iritis, hepatitis, endocarditis and nodular erythema. It is also an important pathogen of secondary infections such as AIDS and leukemia. And it is related to the occurrence of coronary heart disease, which is a serious threat to human health. A common test method is detection of Chlamydia pneumoniae antibodies by ELISA.

Division

Analysis of the detection steps for Chlamydia pneumoniae ELISA

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1. Prepare a washing solution, 1 bottle of concentrated washing solution and 475 ml of distilled water. The prepared washing liquid can be placed in a room temperature environment for a short period of time, and can be stored in a 2-8 ° C environment for a long time.

2. The enzyme conjugate was diluted 1:101, that is, 1 u1 concentrated enzyme conjugate plus enzyme conjugate dilution 1 000 ul (the amount of dilution was determined according to the number of test specimens), and discarded after use.
3. After the kit is equilibrated to room temperature, the specimen to be tested is diluted by 1; 51, that is, 4tA serum is mixed with 200u1 specimen dilution.

4. The diluted specimen was placed in a 37 ° C environment for 20 min.

5. Add 100ul positive, weak positive, negative control and diluted test specimen supernatant in the corresponding wells, add 100u1 specimen dilution to the blank control well, cover the reaction plate, and set at 37 °C for 30min.

6. Drain the liquid in the plate and wash it 5 times with 200-300 ul of washing solution per well.

7. Add 100~1 enzyme conjugate per well, cover the reaction plate, and place it in the environment at 37 °C for 30 min.

8. Drain the liquid in the plate, wash 5 times, and pat dry each time.

9. Add 1 drop of each of the substrates A and B or 50 tzl each, mix and set at 37 ° C for 15 min.
10. Take out, add 1 drop of stop solution, and measure its OD value with a microplate reader at 450 nm. If the naked eye is observed, the stop solution is not added and the result is directly judged.

Precautions:

1. The results of this experiment must be combined with clinical manifestations and medical history to make appropriate clinical treatment.
2. Although the kit contains substances that eliminate interference factors, there may still be a small number of specimens that are difficult to remove. Therefore, if a sample of multiple IgM is positive, RF interference should be considered.